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There is significant variability in lung transplant centers’ approach to HLA antibodies, creating heterogeneity regarding their clinical significance. Some institutions use beads coated with multiple HLA to screen candidate sera and then use single antigen bead (SAB) to determine antibody identity if the pre‐screen is positive. Other centers do not pre‐screen, using SAB alone, which may detect low‐level...
The hyperpolymorphic HLA genes play important roles in disease and transplantation and act as genetic markers of migration and evolution. A panel of 107 B‐lymphoblastoid cell lines (B‐LCLs) was established in 1987 at the 10th International Histocompatibility Workshop as a resource for the immunogenetics community. These B‐LCLs are well characterised and represent diverse ethnicities and HLA haplotypes...
In clinical organ transplantation, flow cytometry crossmatching can be performed on total blood with a hemolysis step or after a preliminary mononuclear cell separation step using a standard Ficoll‐Hypaque protocol. Here, we compared the Ficoll‐Hypaque step with a faster technique for isolating mononuclear cells (the SepMate tube), using the same samples (collected and stored at room temperature for...
The reliability of a scientific work depends on the accuracy of the analysis. Scientific publications in the field of kidney transplantation still contain methodical errors that may lead to wrong conclusions and result in severe consequences for the patients. Using the data from the Collaborative Transplant Study, we are presenting in this study six examples of the erroneous usage of statistical methods...
Recent studies have shown that expression levels of different alleles at the same HLA class I locus can vary dramatically, which might have a broad influence on human disease. However, precise quantification of the relative expression level of each HLA allele is challenging, because distinguishing different alleles on the same locus is difficult. Here, we developed a series of allele‐specific, real‐time...
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